Mitigating effect of Annona muricata Fruit-Extract and Aloe berbadensis miller gel on Aluminum-induced Testicular toxicity in Wistar Rats

Male infertility index due to heavy metal poison has increased over the year in Nigeria. Annona muricata and Aloe barbadensis miller are known for their rich medicinal value. The aim was to investigate the mitigating effect of annona muricata fruit-extract and aloe berbadensis miller gel with the objectives on histology and semen parameters of aluminum-induced testicular toxicity in wistar rats. Twenty-five male rats weighed (100-150g) with five (5) groups were used for the study. Group A: (control): received rats pellet and water. Group B: Given 100mg/kg/d of AlCl3. Group C: Given 100mg/kg/d of AlCl3 and 3ml/kg/d of annona muricata fruit-extract. Group D: Given 100mg/kg/d of AlCl3 and 3ml/kg/d of aloe barbadensis miller gel. Group E: Given 100mg/kg/d of AlCl3 with 3ml/kg/d of annona muricata fruit-extract and3ml/kg/d of aloe barbadensis miller gel. The rats were weighed and sacrificed twenty four hours after the last administration. The results showed that Aluminium treatment induced histological changes in testicular tissue including degeneration of germ cells, lowering of sperm count and increased morphological abnormalities. Animals treated with aluminium along with annona muricata fruit-extract as well as Aloe barbadensis miller showed recovery of normal testicular architecture and improved semen parameters. It is evident that Aluminum Chloride induced deleterious effect on the health of adult wistar rats as illustrated by the distortion of testis histology and semen parameters. Whereas, Sour-sop (Annomia muricata) fruits and aloe Vera gel significantly mitigated the toxic effect of aluminium metal on the reproductive health of male wistar rats.

Key Words: Aloe barbadensis, Annonamuricata, Antioxidant, Spermatogenesis, Toxicity