Microstructure of the Testis and Semen Analysis in Wistar Rats after Treatment with Ethanol Leaf Extract of Acanthus Montanus

Acanthus montanus is a herbal remedy for inflammation and Pains. This work studied the structure of the testes and analysed semen parameters in 21 Wistar rats. Eight weeks old rats weighing l 35-l 50g were used. The leaves were dried, ground and extracted using 70% alcohol. Animals were randomly divided into 3 groups of 7 rats each. The control group was given distilled water. Test groups were administered with ethanol leaf extract of Acanthus montanus, 200mg/kg body weight of rats and 500mg/kg body weight for low and high doses respectively. The administration was done orally once a day with orogastric tube for 14 days. At the end of administration, the animals, at euthanasia with diethyl ether were sacrificed. The testes were dissected out and preserved in 10% buffered formaldehyde. The cauda epididymis was removed and several semen parameters were assessed. Data obtained was subjected to Analysis of Variance (ANOVA) and Pos hoc test (Dunnet's multiple comparison test) for significant differences. Results obtained indicated decreased spermatogenic cells, decreased sperm motility and viability (P<0.05), increased percentage of head abnormality, reduced sperm count but no effect on semen PH and testicular weight. In conclusion Extract altered testicular microstructure and some semen parameters.

Key Words: Acanthus montanus leaf extract, testes, rats