Histological Studies of Effects of Orlistat on the Liver of High Fat Diet Adult Male Wistar Rats

Fats consist of a wide group of compounds, which soluble in organic solvents and insoluble in water. This study is designed to evaluate the impact of high fat diet on the liver of the adult wistar rats. A total number of fifteen wistar rats with an average weight of 130-150g were purchased from the Animal House Handling Unit of Afe Babalola University Ado Ekiti, and divided into 3 groups. Group A was fed with Normal pellet, Group B with High fat Diet only, Group C with high fat diet + Ecoslim (orlistat) for 8 weeks. At the end of the experiment animals were sacrificed and liver of each animal was obtained and set in a buffered 10% formalin solution for histological studies. One-way ANOVA revealed that, there was significant difference in weight change and body mass index (BMI) across all experimental groups (F = 7.450; p = 0.0079). Post hoc analysis showed that weight of high fat fed group was significantly lower than the vehicle treated group. The weight reduction induced by high fat fed was significantly and reversed by orlistart administration (p<0.05). Histological findings revealed alterations in high fat diet alone group which was reversed by orlistat administration in group C. The rats that received High fat diet during the experiment had a higher liver and body weight, with greater nucleus of hepatocytes, as well as an increased visceral fat. High fat diet also caused histometric changes in liver.

Key Words: Histological; Orlistat; High fat diet; Liver