Roles of kolaviron on the prefrontal cortex of adult female Wistar rats exposed to aluminum chloride

Aluminium is a widely recognized neurotoxic agent that can affect the central nervous system and other parts of the body. In this study, effects of kolaviron were investigated on the prefrontal cortex of adult female Wistar rats treated with Aluminium chloride. Twenty – five adult female Wistar rats were purchased and acclimatized for 2 weeks. They were assigned into five groups, each containing five (5) rats, and administered the following respectively; Group A: distilled water, Group B: corn oil, Group C: 200 mg/kg body weight of kolaviron, Group D: 100 mg/kg body weight of Aluminium chloride and Group E: 100 mg/kg body weight of Aluminium chloride + 200 mg/kg body weight of kolaviron. Administration was done consecutively for 14 days with the use of oral cannula. Behavioural assessments (Open field test and Morris water maze) were carried out after which the animals were sacrificed by cervical dislocation and the brain excised. Immunohistochemical techniques using anti-glial fibrillary acidic protein (GFAP) antibody and tumor necrosis factor alpha (TNF-?) were also carried out. Findings revealed that the kolaviron-treated group showed improved learning and memory activities and this was supported by observations in the immunohistochemical assessments following responses to oxidative stress and inflammatory stress markers respectively.

Key Words: kolaviron, aluminium chloride, Morris water maze test, Open field test, GFAP, TNF-?