Histological Evaluation of the Protective Role of Caffeic Acid on the Testes of Adult Wistar Rats Following Exposure to Radio- Frequency Electromagnetic Radiation

Radiofrequency electromagnetic radiation (RF-EMR) has been extensively reported by previous studies to have deleterious effects on the male reproductive system. On the other hand, research has proven that caffeic acid possess potent anti-inflammatory and antioxidant properties. This research aimed to investigate the potential of caffeic acid in mitigating histological damage to the testes caused by RF-EMR exposure. The study assessed testicular histoarchitecture using Haematoxylin and Eosin, as well as Verhoeff-van Gieson stain. The results revealed improved preservation of normal histoarchitecture in the caffeic acid-treated groups compared to the untreated ones, with well-arranged germinal epithelial cells, and improved elastic fibre expression in the peritubular walls of the seminiferous tubules. Pre-treatment with caffeic acid demonstrated its ability to protect the testes of experimental rats from the harmful effects of RF-EMR. The results indicate that treatment with caffeic acid before and after exposure to phone radiation exhibited protective effects on the testes of the rats.

Key Words: radiofrequency, electromagnetic radiation, testes, anti-inflammatory, antioxidant, CAPE